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For cell growth detection, HNSCC cells (1500 cells/well) were seeded into 96-well plates with complete medium. For assessing cell viability to drugs, HNSCC cells (5000 cells/well) were planted into 96-well plates until adherence, and then exposed to CDDP or THL at various concentrations for 24 hours. At the designated time, MTT assay was performed as previously described 22, and IC50 was calculated using GraphPad Prism 6 (La Jolla, USA).
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