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Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis

JL Jianyong Liu
NC Ningjie Chen
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To analyze the functions and pathways associated with DEGs, data were merged to obtain gene symbols, then GO enrichment analysis and KEGG pathway analysis were performed by using enrichGO function and enrichKEGG function of clusterProfiler package of R [19], respectively. Subsequently, GO enrichment results were visualized by using a GOChord function in GOplot package [20], and KEGG enrichment results were visualized by using a Barplot function in clusterProfiler package, independently. The GO included molecular function, biological process, and cellular component. The P < 0.05 was regarded as statistically significant.

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