2.8. Quasi H-Score Calculation

The percentage of samples with low, medium, and high protein expression for the HPA datasets was determined (only for genes without high protein expression across the critical normal tissues), and the quasi H-score was calculated per tumor type based on the following formula (1):

Quasi H − score (range, 0 − 300) = (1 × percentage of patients with low protein expression) + (2 × percentage of patients with medium protein expression) + (3 × percentage of patients with high protein expression)(1)

All surface protein-coding genes with a quasi H-score above the threshold (≥150) in at least one tumor type were considered as potential ADC targets. The cut − off ≥ 150 was defined based on the previous studies utilizing the H-score [16–18].