Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Next-generation impactor (NGI) studies

RK Ranjot Kaur
SD Sarah R. Dennison
AB Andrea J. Burrow
SR Shivaprakash M. Rudramurthy
RS Rajan Swami
VG Varun Gorki
OK O. P. Katare
AK Anupama Kaushik
BS Bhupinder Singh
KS Kamalinder K. Singh
ask Ask a question
Favorite

Particle deposition studies of LPH NPs dispersion were performed employing an NGI at a flow rate of 15 L. minute−1. Sidestream nebulizer was attached to the induction port of an NGI (M/s Copley Scientific, UK) using a mouthpiece adapter and filled with 10 mL of LPH NPs dispersion with an aerosol collection time of 5 min [39]. After completing the measurement, the cup holder tray was taken out, and each stage of the impactor was washed with 10 mL of extraction solvent (methanol and 1% acetic acid solution), filtered and subjected to HPLC analysis for determining the voriconazole content. Mass median aerodynamic diameter (MMAD) and geometric standard deviation (GSD) were computed employing online MMAD calculation software [40]. Other parameters, like fine particle fraction (FPF) and emitted dose (ED), were also calculated as per the standard formulas [41].

Copyright and License information: The Author(s) ©2021
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A