Balance Beam Test

The ability of the mice to keep balance was evaluated in the balance beam test. Balance beam test was performed as previously described ⁵⁰. Mice were placed on the same starting point of a horizontal wooden bar (0.9 × 0.9 × 50 cm) 40 cm above the ground and a dark goal box on destination to attract the mouse to run up to this dark and safe environment. The time taken to cross the beam was measured. Mice were given a session and recorded three times per day with a 15-20 min interval and repeated for five successive days.