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We measured the cell proliferation rate by CCK-8 kit as previously described. ESCC cells treated with siRNAs were collected and seeded into a 96-well plate at a density of 2000 cells/well. Next, we added 10 μl CCK-8 reagent into each well after culturing for 24, 48, 72, 96 h. We detected the absorbance at 450 nm after incubation CCK-8 reagent for 2 h at 37 °C. Data were collected by a microplate reader for statistical analysis.
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