The full length of CDS of APC with His tag was subcloned into pcDNA 3.1 vector (Thermo), the full length of CDS of FAM83B with Flag tag was subcloned into pcDNA 3.1/CAT. Two vectors were co-transfected into COV362 cells using Lipofectamine 3000 (Thermo). Co-IP were performed according to the previous reports15.
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