Cell proliferation viability assay

ZD Zhaoming Deng
WL Wei Liao
WW Wei Wei
GZ Guihua Zhong
CH Chao He
HZ Hongbo Zhang
QL Qiaodan Liu
XX Xiwei Xu
JL Jun Liang
ZL Zhigang Liu
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Cell counting kit 8 (CCK-8) (MedChemExpress, USA) was adopted to examine the cell proliferation viability. Cells were seeded in a 96-well plate with a density of 2000 cells per well. To find out an optimal toxicity effect on the cancer cells, Anlotinib with various concentration was added 24 h after seeding in the plate of each cell lines before CCK-8 (1:10 diluted in 100 µL medium per well) was added in each plate 24, 48 and 72 h later. After incubation of the mixture for 3 hours, measurement of Formazan generated was carried out at 450 nm using a microplate reader (Molecular Devices CMax Plus, USA).

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