ADAM17 activity was assayed by the release of the quenched fluorescence group from a specific FRET substrate (BioVision, Milpitas, CA). The whole cell lysate was aliquoted (20 μg/well) in commercial assay buffer. After a short incubation at 37 °C, the FRET substrate was added to each well. The fluorescence generated by substrate hydrolyzation was then recorded kinetically for 60 min at 318/449 nm (Ex/Em).
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