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Sample examination

FY Fuat Yavrum
UE Ufuk Elgin
ZK Zeynep Adiyaman Kocer
VF Vildan Fidanci
ES Emine Sen
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All serum samples were taken from the antecubital vein just before surgery and then transferred to a biochemistry tube containing a separator gel. After the blood samples were sent to the laboratory, they were centrifuged (Eppendorf 5810, Hamburg, Germany) at 3000 rpm for 10 min, and the obtained serum stored at − 80 °C (SANYO MDF U6186, Osaka, Japan) until analysis.

The AH samples were obtained using a 1 ml syringe immediately after entering the anterior chamber, and all samples collected during surgery were obtained before any cataract-surgery related medications were applied. The samples were stored at − 80 °C until analysis. The concentrations of clusterin were assessed using a Human Clusterin PicoKine ELISA Kit (MyBioSource, Catalog No. MBS176539, LOT 5601235403, CA, USA) according to the manufacturer’s instructions. The author performing the clusterin analyses (VF) was blinded to patient details. Anterior chamber concentration units were μg/ml, those for serum samples were ng/ml.

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