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VSL #3

ZD Zach Dwyer
MC Melany Chaiquin
JL Jeffrey Landrigan
KA Kiara Ayoub
PS Pragya Shail
JR Julianna Rocha
CC Christie L. Childers
KS Kenneth B. Storey
DP Dana J. Philpott
HS Hongyu Sun
SH Shawn Hayley
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Each morning one packet of freeze dried, unflavored VSL#3 (450 x 109 CFU), commercially purchased from Ferring Canada, was dissolved in 250 mL of room temperature non-sterile tap water. Ten milliliters was then placed into each individual water tube. Tubes were labeled, weighed, and given to animals. When tubes were replaced the following day with fresh VSL#3, they were again weighed and the difference taken to determine the quantity ingested. Animals drank an average of 3 mL a day, and this did not differ between the groups. Thus, all mice received (1.8 x 109 CFU/ml x 3 ml) approximately 5.4 x 109 CFU per day. This dosage is well within the range of previous studies that used a mouse model of colitis; 4 x 109 CFU/dose over a 7-day interval or 3 × 108 CFU VSL#3 for a period of 28 days [39, 40]. But these studies used oral gavage, whereas we presently avoided using oral gavage owing to its inherent stressful impact.

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