293T-ASC-GFP cells were described previously8. All other 293T-GFP responder cell lines were constructed by lentiviral transduction (pCDH vector backbone, System Biosciences). All transient expression plasmids were cloned into the pCS2+ vector using standard restriction cloning. Polyclonal Cas9/CRISPR knockout cell lines were generated with lentiCRISPR-v2 (Addgene #52961) and selected with puromycin. Knockout efficiency was tested with western blot 7–10 days after puromycin selection. Site-directed mutagenesis was carried out with QuickChangeXL II (Agilent #200522).
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