Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Cytometric Bead Array (CBA) analysis

GL Guohua Lou
AL Aichun Li
YC Yelei Cen
QY Qin Yang
TZ Tianbo Zhang
JQ Jinjin Qi
ZC Zhi Chen
YL Yanning Liu
request Request a Protocol
ask Ask a question
Favorite

Cytokine levels in the serum samples were detected by a BD LSR Fortessa cytometer (Becton Dickinson Holdings Pte Ltd, Singapore) using a commercially available BD™ Cytometric Bead Array (CBA) Mouse Inflammation Kit (BD Biosciences, USA) following the manufacturer’s instructions [12]. In brief, cytokine standards were prepared using a vial of lyophilized mouse inflammatory cytokines, including IL-6, IL-10, monocyte chemoattractant protein-1 (MCP-1), interferon-γ (IFN-γ), TNF-α, and IL-12p70, and assay diluent by serial dilutions. Capture beads were added to each tube containing samples, standards, and negative controls and incubated for 30 min at room temperature in the absence of light. The flow cytometer was calibrated using cytometer setup beads, and the assay was performed.

Copyright and License information: The Author(s) ©2021
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A