Novel object recognition test

WH Wan Yun Ho
IA Ira Agrawal
ST Sheue-Houy Tyan
ES Emma Sanford
WC Wei-Tang Chang
KL Kenneth Lim
JO Jolynn Ong
BT Bernice Siu Yan Tan
AM Aung Aung Kywe Moe
RY Regina Yu
PW Peiyan Wong
GT Greg Tucker-Kellogg
EK Edward Koo
KC Kai-Hsiang Chuang
SL Shuo-Chien Ling
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Recognition memory was assessed using the novel object recognition test paradigm. Animals were exposed to the testing arena for two consecutive days. On the third day, animals were presented with two training objects for 10 min. Probe tests were carried out 20 min, 16–24 h and 10–14 days after the completion of training, for short-term (STM), long-term (LTM) and remote (RM) memory respectively. For probe tests, animals were returned to the arena for 10 min, where they were presented with one training object and one novel object. The sets of objects used were different for each of the assessed ages. Video recordings of the interactions with the objects were taken and analyzed by Topscan (Cleversys Inc, Reston VA). The data are presented as the interaction time that was spent with the novel object over total interaction time.

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