Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Alizarin red staining (mineralization)

SP Stephan Payr
ER Elizabeth Rosado-Balmayor
TT Thomas Tiefenboeck
TS Tim Schuseil
MU Marina Unger
CS Claudine Seeliger
MG Martijn van Griensven
ask Ask a question
Favorite

Alizarin red staining and measurement were performed to measure mineralization. Cultured cells were fixed with ice-cold ethanol 96% for 30 min in a 24-well plate. After washing twice with dH2O, 400 μl alizarin red staining solution was added in each well for 10 min, washed again four times. The stain was eluted for 20 min into a solution of 10% hexadecylpyridiniumchloride (Sigma-Aldrich). Absorbance was read at 562 nm. Alizarin red concentration for samples was calculated according to standard curve and results were normalized to the protein content analysed via SRB staining [23].

Copyright and License information: The Author(s) ©2021
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A