The animal experiments were approved by the local Institutional Animal Care and Use Committee at Yale University (New Haven, Connecticut) and conducted in accordance with the United States Public Health Service’s Policy on Humane Care. Twelve female Sprague–Dawley rats (Charles River Laboratories, Wilmington, MA, USA) between the ages of 12–16 weeks were used. All rats received standard rat chow and water ad libitum and were housed in standard conditions in a 12 h light/dark cycle.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.