Athymic BALB/c nude mice (6-week-old) were housed in laminar air flow cabinets under pathogen-free conditions. A549 or H460 cells (1 × 106) with stably overexpressing lnc-GAN1 or control vector were injected into the flank of a mouse (n = 6 per group). After 24 days, the mice were sacrificed under anesthesia, and the tumor tissues were harvested and weighted. The maximum diameter and width of tumors were measured with a caliper. Tumor volume was calculated with the following formula: Volume = (length × width2)/2. Lnc-GAN1 levels in the tumors were determined by qRT-PCR [25]. The animal experiments were approved by the Animal Care and Use Committee of Sun Yan-Sen University Cancer Center.
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