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Optimal temperature and thermostability of lipase activity
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Optimum temperature was assayed by measuring activity at optimum pH with varying temperature (20–90 °C), and the thermostability of the lipase enzyme was determined by incubating the partially purified enzyme solution at the temperatures range for 30–90 min. The relative lipase activity was measured according to the standard assay conditions. The activity of untreated enzyme (control) was taken as 100% [30].
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