Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Colorimetric assays

JD Jinrui Dong
SV Sivakumar Viswanathan
EA Eleonora Adami
BS Brijesh K. Singh
SC Sonia P. Chothani
BN Benjamin Ng
WL Wei Wen Lim
JZ Jin Zhou
MT Madhulika Tripathi
NK Nicole S. J. Ko
SS Shamini G. Shekeran
JT Jessie Tan
SL Sze Yun Lim
MW Mao Wang
PL Pei Min Lio
PY Paul M. Yen
SS Sebastian Schafer
SC Stuart A. Cook
AW Anissa A. Widjaja
request Request a Protocol
ask Ask a question
Favorite

Alanine aminotransferase (ALT) activity in the cell culture supernatant and mouse serum was measured using ALT Activity Assay Kit (ab105134, Abcam). Cellular and liver glutathione (GSH) levels were measured using Glutathione Colorimetric Detection Kit (EIAGSHC, Thermo Fisher). Total hydroxyproline content in mouse livers was measured using Quickzyme Total Collagen assay kit (QZBtotco15, Quickzyme Biosciences). The levels of triglycerides in hepatocyte lysates and in mouse serum and livers were measured using Triglyceride Assay Kit (ab65336, Abcam). Mouse serum levels of aspartate aminotransferase (AST), cholesterol, and β-hydroxybutyrate were measured using AST Assay Kit (ab105135, Abcam), Cholesterol Assay Kit (ab65390; Abcam), and beta-hydroxybutyrate (Ketone body) Colorimetric Assay Kit (700190; Cayman Chemicals), respectively. All colorimetric assays were performed according to the manufacturer’s protocol.

Copyright and License information: The Author(s) ©2021
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A