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Il11ra-floxed mice (CKO)

JD Jinrui Dong
SV Sivakumar Viswanathan
EA Eleonora Adami
BS Brijesh K. Singh
SC Sonia P. Chothani
BN Benjamin Ng
WL Wei Wen Lim
JZ Jin Zhou
MT Madhulika Tripathi
NK Nicole S. J. Ko
SS Shamini G. Shekeran
JT Jessie Tan
SL Sze Yun Lim
MW Mao Wang
PL Pei Min Lio
PY Paul M. Yen
SS Sebastian Schafer
SC Stuart A. Cook
AW Anissa A. Widjaja
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Il11ra-floxed mice, in which exons 4–7 of the Il11ra1 gene were flanked by loxP sites, were created using the CRISPR/Cas9 system as previously described42. To induce the specific deletion of Il11ra1 in hepatocytes, 6–8-week-old male homozygous Il11ra1-floxed mice were intravenously injected with AAV8-Alb-Cre virus (4 × 1011 gc); a similar amount of AAV8-Alb-Null virus were injected into homozygous Il11ra1-floxed mice as controls. The AAV8-injected mice were allowed to recover for 3 weeks prior to HFMCD, WDF, or NC feeding. Knockdown efficiency was determined by western blotting of hepatic IL11RA.

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