Formalin-fixed, paraffin embedded liver sections were deparaffinized and stained with hematoxylin and eosin (H&E) to assess liver histology or with Picrosirius Red (Sigma, 365548) / Fast Green (Sigma, F7258) for assessment of fibrosis. Stained sections were provided to an expert liver pathologist for evaluation of disease severity utilizing the NAFLD activity score (NAS) and fibrosis stage according to the NASH-CRN scoring system [25]. The pathologist was blinded to the study and treatment arms.
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