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Virus Virulence and Cytotoxicity Assay

YZ Yun-Li Zhao
ZG Zhong-Ping Gou
JS Jian-Hua Shang
WL Wan-Yi Li
YK Yu Kuang
ML Ming-Yuan Li
XL Xiao-Dong Luo
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The anti-RSV and anti-HSV-1 activities of TA were determined by the titer reduction assay as previously described [57]. Briefly, Hep2 (to RSV) and Vero cell monolayers (to HSV-1) were co-incubated with a series of virus concentrations from 10–3 to 10–13. The cytopathogenic effect (CPE) was observed and half of tissue culture infective dose (TCID50) was calculated according to Reed & Muench formula [58].

The half toxicity (TC50) and non-cytotoxic concentration (TC0) of TA against Hep2 and Vero cells were determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay as previous [59].

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