Preparation of the Pometia pinnata extract

P. pinnata leaf extract (PPLE) used on the ablation process was collected from The University of Indonesia campus in February. They were surface cleaned with running tap water to remove debris and other contaminated organic contents, followed by double distilled water and air-dried at room temperature. About 10 g of finely cut leaves were kept in a beaker containing 100 mL of the solution and boiled for 30 min. The leaf was extracted with three different solutions in this experiment: double distilled water and ethanol–water with a concentration of 20–40%. The extract was cooled down and filtered with filter paper no.1, and the extract was stored at 4 °C for further use. A phytochemical screening analysis was performed for detecting the compound consisted of P. pinnata leaf extract. The extract was tested for phytochemical content qualitatively to determine the presence of alkaloids, terpenoids, flavonoids, saponins and phenols.[36,37].