Quantification of mitochondrial morphology

MitoTracker® Mitochondrion-Selective Probe (Invitrogen, Gaithersburg, MD) was used to detect the morphology of mitochondria. Cells were incubated with the staining solution containing MitoTracker® probe (100 nM) at 37°C for 30 min and fixed with 4% formaldehyde, followed by DAPI staining. The images of each well were obtained in three different fields by confocal microscopy. The morphology of mitochondria was analyzed using Image J with mitochondrial network analysis toolset (MiNA).