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Transmission electron microscope (TEM)

HH Hui-Fang Hu
WX Wen Wen Xu
YL Yang-Jia Li
YH Yan He
WZ Wei-Xia Zhang
LL Long Liao
QZ Qi-Hua Zhang
LH Lei Han
XY Xing-Feng Yin
XZ Xiao-Xu Zhao
YP Yun-Long Pan
BL Bin Li
QH Qing-Yu He
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The cells were fixed with TEM fixative (Wuhan Servicebio Technology, Wuhan, China) at 4°C for 4 h, then pre-embedded in 1% agarose, and fixed with 1% osmium tetroxide. After dehydration at room temperature using ethanol, the cells were embedded in Poly/Bed 812 resin, followed by polymerization at 65°C. The ultrathin section was stained with 2% uranium acetate saturated alcohol solution and 2.6% lead citrate. TEM (HT7700, HITACHI, Fukuoka, Japan) was used to take images for morphological analysis.

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