Validation of RNA sequencing expression of central genes

Gene Expression Profiling Interactive Analysis (GEPIA; http://gepia.cancer-pku.cn/index.html) and GEPIA2 (http://gepia2.cancer-pku.cn/#index) were the functional tools for analyzing the prevalence and expression of a gene signature in the Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) samples ¹⁸. To further validate the intersecting DEGs, GEPIA and GEPIA2 were used to probe the RNA sequencing expression between BC and normal breast tissues. P<0.05 was considered significant.