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2.4. Optimization of simultaneous extraction

IB Isaac Bowers
BS Bikram Subedi
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Raw wastewater samples (n = 3) were centrifuged and filtered as described in section 2.2., spiked with a mixture of target analytes (125 ng each), internal standards, and extracted as described above. One wastewater sample (not spiked with analytes) was included to determine the background concentration of target isoprostanes in wastewater and one ultrapure water sample was included to evaluate the procedural contamination. Moreover, one wastewater sample spiked at 125 ng of each target analyte was extracted using Oasis® MCX mix-mode cation exchange (6 cc, 150 mg) cartridge. All extracts were concentrated and adjusted the final volume to 500 μL using methanol and analyzed.

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