Animal protocols were in accordance with the “Animal Care and Committee Guidelines of the Third Military Medical University.” For the subcutaneous xenograft tumor models, male athymic nude mice (4 to 6 weeks old) were used. GFP-labeled HL-60 cells (5 × 106) were suspended in Dulbecco’s minimum essential medium with the same volume of Matrigel (BD Biosciences) and subcutaneously injected into the flank of each mouse. For mouse model of human AML, male C57BL/6 mice (6 to 8 weeks old) were exposed to a sublethal irradiation at a dose of 6 Gy using a cobalt-60 source. GFP-labeled HL-60 cells (5 × 106) suspended in 300 μl of PBS were injected into mice through the tail vein. GFP-labeled malignant cells in peripheral blood were monitored by IVFC for 20 days, and leukemia blasts infiltrated in peripheral blood, bone marrow, spleens, and liver were detected to confirm that leukemia mouse models were established successfully. NOD/SCID mice engrafted with GFP-labeled human AML cells by tail vein injection were used to detect anti-leukemia efficacy of IR-26 in vivo.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.