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Confirmation of transgenic chrysanthemum
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Putative transgenic plantlets of Jinba and C029 were rooted in Murashige and Skoog solid media supplemented with 0.2 mg L−1 NAA and 20 mg L−1 kanamycin. The positive plants were screened via PCR and RT-qPCR using the primers NptII-F/R and qRT-CmWRKY15-1-F/R (Supplemental Table 1).
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