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Measuring phenolic acid and anthocyanin contents

WZ Wei Zhou
MS Min Shi
CD Changping Deng
SL Sunjie Lu
FH Fenfen Huang
YW Yao Wang
GK Guoyin Kai
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Hairy roots grown in shaker flasks were harvested and dried in an oven. The dried roots were ground to a powder, after which 200 mg samples were extracted with 16 mL of methanol/dichloromethane (3:1, v/v), sonicated for 1 h, and centrifuged at room temperature. Phenolic acid was extracted from the samples for HPLC analysis, as described previously5,12,14. The phenolic acids Sal A, Sal B, CA, and RA were extracted from the selected transgenic lines and quantified by comparison with standard curves and the corresponding retention times. Total phenolic acids were designated as TPA. Anthocyanin contents were determined by spectrophotometry by measuring the absorbance at 530 nm and 657 nm, with solutions without extracts used as blank controls39.

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