Subcellular localization of SmMYB1

The full-length ORF of SmMYB1 (without the stop codon) was fused to a green fluorescent protein (GFP) gene under the control of the CaMV 35S promoter within a pMON530 vector to generate a SmMYB-GFP fusion protein as described previously^4,5 (Supplementary Fig. S1). The constructs were subsequently transferred into Agrobacterium tumefaciens GV3101, which were then infiltrated into five-week-old N. benthamiana leaves for transient transformation, as described previously⁵. pMON530-GFP was used as a control. To visualize the nuclei, 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) solution (10 μg/mL) was injected into N. benthamiana leaves with a syringe 3 h prior to microscopy observations. YFP and DAPI signals were excited at 488 and 405 nm, respectively. At 48 h after infiltration, the GFP signals were visualized using a confocal spectral microscope imaging system (Leica TCS SP5).