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Transformation of A. thaliana

BL Bin Liu
SZ Shuo Zhao
PL Pengli Li
YY Yilu Yin
QN Qingliang Niu
JY Jinqiang Yan
DH Danfeng Huang
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Transformation assays were performed as previously described31. To generate CsGA2ox8.1 and CsGA2ox8.2 overexpression constructs, full-length CsGA2ox8.1 and CsGA2ox8.2 cDNAs were cloned and inserted into a pBI121 vector between the XbaI and SmaI restriction sites40. The constructs were then introduced into Agrobacterium tumefaciens strain LBA4404 by electroporation, which were then transformed into wild-type A. thaliana (Col-0), as previously described41. The transgenic plants were screened on MS media supplemented with 50 mg/L kanamycin and then confirmed via PCR. The primers used are listed in Supplementary Table S3.

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