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LSL-KrasG12D/+ Trp53fl/fl mouse model.

JG Jiawei Guo
YL Yanan Liu
JL Jing Lv
BZ Bin Zou
ZC Zhi Chen
KL Kun Li
JF Juanjuan Feng
ZC Zhenyu Cai
LW Lai Wei
ML Mingyao Liu
XP Xiufeng Pang
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LSL-KrasG12D/+Trp53fl/fl (B6.129-Krastm4Tyj Trp53tm1Brn/J) mice on a C57BL/6J background were purchased from The Jackson Laboratory. Initially, 8-week-old mice were anesthetized with isoflurane via a gas chamber and infected with 5 × 107 infectious particles of Ad-Cre (HanBio) per mouse by intranasal injection. Mice were randomly divided into 2 groups 8 weeks after the operation. Mice were treated with vehicle and OTX015/FX1 daily for 3 weeks. Next, mice were allowed to recover in the untreated condition for an additional week. On day 28, tumors were measured by micro-CT (PerkinElmer).

Copyright and License information:  ©2021 American Society for Clinical Investigation

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