AR symptom score

As previously described ²¹, the nasal symptom score was based on three features, each graded on a four-point scale score between 0 and 3: (i) sneezing: 0, none; 1, 1-3 sneezes per 20 min; 2, 4-10 sneezes per 20 min; 3, ≥11 sneezes per 20 min; (ii) nasal mucus: 0, none; 1, mucus in nostrils; 2, mucus outflow from nostrils; 3, mucus outflow to face; and (iii) nasal itching: 0, none; 1, nose rubbing 1-2 times per min; 2, nose rubbing 3-5 times per min; 3, nose rubbing >6 times per min. The three scores were added to give a total score between 0 and 9 points. Animals recording a total score of ≥5 points were considered to have AR (Table ​(Table1).1). The symptom scores were measured every morning after the OVA challenge from day 21 to 55, and the observation time for symptom scoring is 20 minutes from the moment of OVA challenge.

Nasal symptom scoring in the AR mouse model