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ROS production was measured by staining with 2',7'-dichlorofluorescin diacetate (DCFDA, Sigma) according to the manufacturer's instructions. In brief, BMDCs from WT mice were infected with P. nigrescens at MOI 1, 10, and 100 for 24 h. The cells were washed twice with PBS, and then incubated with 15 μM DCFDA for 10 min at 37°C. Subsequently, the cells were washed with PBS and detected using a fluorescence microscope.
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