Exhaustive swimming exercise

Endurance performance is an important parameter for evaluating anti-fatigue, evaluated by an exhaustive swimming test. On the 16th day of the experiment, 30 minutes post MMP administration, each animal was submitted to exhaustive swimming test with intensity equivalent to overload of 5% of the body weight tagged to the tail, then was evaluated in a columnar swimming pool maintained at 28±1°C, the swimming pool is 65 cm high, 40 cm diameter, 40 cm deep. Each mouse was loaded with a lead block, weighting approximately 5% of the body weight tagged to the tail. The endurance performance of each mouse was measured as the swimming time, recorded from the beginning to exhaustion. The mice were determined to be exhausted when uncoordinated movements occurred with failure to swim to the surface within a 7-second period ²⁴. The exhaustive swimming time was used as an index of exercise endurance.