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Determination of serum cytokine levels

XF Xixia Feng
LC Lu Chen
RZ Ruihao Zhou
XB Xiuqun Bao
HM Hongxia Mou
LY Ling Ye
PY Pingliang Yang
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At the end of the behavior test, we anesthetized the rats and fully exposed their hearts. Then, we centrifuged the whole blood from the right atrium for 20 minutes and refrigerated the upper serum at -80°C. The serum IL-1β, IL-6, and TNF-α levels were measured via a commercial ELISA kit (BD Biosciences Co., San Jose, CA, USA) according to the manufacturer's protocol.

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