Hematoxylin and eosin (H&E) staining was performed following a standard procedure. Eight minutes after FeCl3 treatment, the arteries were fixed in 10% formalin for 24 h at 4°C, then washed and embedded in paraffin. The paraffinized segments were sectioned, deparaffinized, rehydrated, and stained with H&E. The sections were digitally imaged under a microscope (BA210 Series; Motic, Richmond, BC, Canada).
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