Tumor xenograft model

Equal volumes of suspensions of MG63, MG63-R1, MG63-R1-CtBP1-KD1, MG63-R1-FOXM1-KD1, MG63-R2, MG63-R2-CtBP1-KD1, and MG63-R2-FOXM1-KD1 cells were injected subcutaneously into six-week-old C57BL/6 mice (n=10 for each cell line). The tumor width and length were measured at 5-day intervals and tumor volumes were determined using the formula: volume = (length × width²)/2. The effects of NSM00158, NSC95397, and RCM1 on inhibition of tumor growth were examined by subcutaneous injection of equal volumes of MG63 and MG63-R1 cell suspensions into six-week-old C57BL/6 mice (n=50 for each cell line). Small molecules were injected into mice (n=8 for each small molecule) at different dosages (NSM00158: 2 mg/kg; NSC95397: 6 mg/kg; and RCM1: 1.7 mg/kg) every five days when the tumor volumes had reached approximately 200 mm³. Tumor volumes were also determined at 5-day intervals. The effects of NSM00158, RCM1, CDDP, NSM00158+CDDP, and RCM1+CDDP were evaluated by subcutaneous injection of MG63-R1 cell suspension into six-week-old C57BL/6 mice (n=50). CDDP (5 mg/kg) was combined with or without NSM00158 (2 mg/kg) and RCM1 (1.7 mg/kg), followed by injecting into mice (n=8 for each small molecule) when tumor volumes had reached approximately 200 mm³. The tumor volumes were determined at 5-day intervals. All animal experiments were performed following a protocol reviewed by the Institutional Animal Care and Use Committee (IACUC) of Honghui Hospital in Xi'an Jiao Tong University.