Brain atrophy volume measurement

Mouse brains were perfused with 0.1 mol/L PBS and then with 4% paraformaldehyde (PFA). Brain samples post-fixed overnight in 4% PFA were dehydrated in 30% sucrose until they sank to the bottom of the liquid and then stored at -80 °C. Subsequently, the samples were cut into 30 μm sections from the anterior commissure to the hippocampus and stained with 1% cresyl violet. The atrophy area ΔS was calculated as the contralateral area minus the ipsilateral area, and the atrophy volume was calculated as: V = h / 3 [ ΔSn + + ΔSn+1], where “h” represents the thickness between the two sections ²³.