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Alexander staining for pollen viability
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Anthers from flowers at stage 13 (Smyth et al., 1990) were incubated in Alexander’s stain (Alexander, 1969) on objective slides covered with coverslips at room temperature for 20 hr. Bright field images were taken using a Zeiss Axio Imager M1 microscope. Adjustment of brightness and contrast of whole images was done with Fiji.
Copyright and License information: ©2020, Lupanga et al
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