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Total RNA was extracted from cells using TRIzol reagent (Invitrogen, Carlsbad, CA). Reverse transcription and Q-PCR analysis were performed using an Eppendorf Realplex2 (Peng et al., 2009). Primers for Q-PCR analysis are listed in Supplementary file 1.
Copyright and License information: ©2014, Zhu et al
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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