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For LT-HSC methylcellulose colony assays, 40 double-sorted LSK CD150+CD48ā cells from Lsd1fl/fl or Lsd1fl/fl Mx1Cre (pre-poly(I:C)) animals were plated per 1.1 ml of Methocult M3434 (Stem Cell Technologies, Vancouver, Canada) containing 1000 U/ml mouse interferon alpha (R&D Systems, Minneapolis, MN) to activate Mx1Cre-mediated deletion in vitro. Single colonies were picked and PCR genotyped to verify Lsd1 deletion.
Copyright and License information: ©2013, Kerenyi et al
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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