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Median tissue culture infectious dose (TCID50) assay

KC K-H. Chan
SS S. Sridhar
RZ R.R. Zhang
HC H. Chu
AF A.Y-F. Fung
GC G. Chan
JC J.F-W. Chan
KT K.K-W. To
IH I.F-N. Hung
VC V.C-C. Cheng
KY K-Y. Yuen
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Confluent Vero E6 cells on 96-well plates were incubated with 100 μL of serial 10-fold dilutions of virus in MEM containing 1% FBS for 1 h at 37°C. Next, the virus was removed from the 96-well plates and 100 μL of fresh MEM with 1% FBS was added to the cells. After the change of medium, cells infected with SARS-CoV-2 underwent a 5-day incubation period and cells infected with SARS-CoV underwent a 3-day incubation period; the cytopathic effect was recorded. Median tissue culture infectious dose (TCID50) was determined by the Reed and Muench method [8].

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