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Quantification of intracellular acetyl-CoA was performed using the PicoProbe Acetyl-CoA Fluorometric Assay Kit (catalog no. K317-100, BioVision, USA) according to the manufacturer’s instructions. hPTCs were incubated for 48 hours with 100 nM CsA, 100 nM cisplatin, and vehicle for control. Cells were lysed on ice, and fluorescence was measured with a plate reader (PerkinElmer, USA).
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