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Plaque assays were performed using the MDCK London cell line, as previously described (36), with the exception that cells were incubated with the agar overlay for 48 hours. Plaques were counted, and the final mAb concentration that reduced the number of plaques to 50% was determined using GraphPad Prism software. The assay was performed in duplicate two to three times.
Copyright and License information: ©2020
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