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Tumor samples were obtained and slides were stained as previously described (3). Briefly, staining was performed at the UCLA Translational Pathology Core Laboratory using Leica Bond ancillary reagents and REFINE Polymer DAB detection system. Briefly, slides were stained with S100 (Dako), CD8 (Dako), PD-L1 (clone SP142, Spring Bio), and MHC I (clone HC 10, Sapphire NA) and scanned at 40× on an Aperio ScanScope AT (Leica Biosystems).
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