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Cell viability was measured using CellTiter-Glo (Promega Corp.). Single cells were seeded and treated in triplicates in opaque 96-well plates in a total volume of 100 μl. The neuroblastoma cell lines SK-N-BE(2)c, SK-N-SH, SK-N-FI, and IMR-32 were incubated for 24 hours before treatment to allow attachment to the plate. PDX cells were treated directly after seeding. After 72-hour treatment, 50-μl CellTiter-Glo reagent was added to each well, and the luminescence was measured using a Synergy2 plate reader (BioTek) and Gen5 software (BioTek).
Copyright and License information: ©2020
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