Cell viability assay

KB Krzysztof Bielawski
KL Katarzyna Leszczyńska
ZK Zbigniew Kałuża
AB Anna Bielawska
OM Olga Michalak
TD Tamara Daniluk
OS Olga Staszewska-Krajewska
AC Anna Czajkowska
NP Natalia Pawłowska
AG Agnieszka Gornowicz
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The growth inhibitory effects of the test compounds against human skin fibroblast cells were measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Compounds were dissolved in dimethyl sulfoxide (DMSO) and screened at a range of concentrations against normal cells. The cytotoxic activity was determined after 24 h. All experiments were repeated at least three times.

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