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Intimal RNA isolation from aorta tissue
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Isolation of intimal RNA from aorta was performed as previously described in (54, 55). Briefly, aortas were carefully flushed with phosphate-buffered saline (PBS), followed by intima peeling using TRIzol reagent (Invitrogen). TRIzol was flushed for 10 s, followed by a 10-s pause, flushed another 10 s, collected in an Eppendorf tube (~300 to 400 μl in total), and snap-frozen in liquid nitrogen.
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